Louis, Missouri, USA. Bacterial subcellular fractions were obtained according to the method described by Morton et al 11 in , with some modifications. Protein concentration was determined by the method of Bradford Rabbit antiserum Two New Zealand rabbits weighing 2 kg each were intramuscularly inoculated with A. After 7 d, the rabbits were given intramuscular booster inoculations without adjuvant 3 times, at 1-wk intervals. A titer of for the antibody against A.
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Faushura The values of each point represent the average and standard error. Rabbit antiserum Two New Zealand rabbits weighing 2 kg each were intramuscularly inoculated with A. These results suggest that the kDa protein could be specific to A. The authors declare that there is no conflict of interests regarding the publication of this paper. It is nonmotile and non-sporulated.
Urine collection was performed by blocking the breath for 30 seconds. Severe testicular degeneration may impact directly on fertility of the animal. In addition, frequency of detection of A.
Molecular aspects of some virulence factors of Haemophilus sommus. Thickening and fibrous adherence of tunica albuginea with tunica vaginalis were also frequent changes Figure 6 d. Whole blood was collected by jugular vein puncture with vacuum collection system. Production and preliminary characterization of monoclonal antibodies to Actinobacillus sp.
They also provide an excellent source for the purification and characterization of biologically active molecules. However, other isolates identified as Actinobacillus spp. The owner of a mob of mixed age first cross ewes reported that weeks prior to lambing due to start mid Septemberseven ewes aborted or produced stillborn premature lambs.
Microscopic findings in rams experimentally infected by Histophilus somni. Specific protein of A. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Some aspects of epididymitis of rams in New Zealand.
However, it is still necessary to conduct tests with serum from animals naturally infected with microorganisms other than A. The isolate was PCR positive for A. There are rare reports of A. Actinobacillus seminis — Wikipedia Among the etiologic agents of epididymitis are several bacterial species, such as Brucella ovis, Actinobacillus seminis, Mannheimia haemolytica Pasteurella haemolyticaPasteurella multocida, and Histophilus somni Haemophilus somnus 3. Brucella ovis was reported as the main etiologic agent in Australia in ; however, A.
Membrane vesicles released by Actinobacillus pleuropneumoniae contain proteases and Apx toxins. Support Center Support Center. Gross and microscopic findings in both experimental infections were similar to those seen in cases of natural infection [ — 26 ]. The absence of inflammatory reaction and bacterial isolation from only two testes of all infected animals indicate that A. This disease is a serious reproductive disorder, with marked pathological changes in the genital tract 2.
The lesions observed in the body of the epididymis were multifocal mononuclear interstitial or perivascular infiltrate and small spermatic granulomas Figure 8 c. Comparison and partial characterization of the protein profiles and outer membrane antigens of Actinobacillus species isolated from ram lambs with epididymitis. No protein of these bacteria cross-reacted with the kDa protein of A. Whole cells and each subcellular fraction of A.
Microscopically, bacterial epididymitis initially elicits a neutrophilic inflammation that can be followed by epididymal epithelial hyperplasia or metaplasia, duct obstruction with content retention. The length was measured in dorsoventral and width semonis craniocaudal direction.
Ovine brucellosis caused by B. In the Australian context, epididymitis is the most important manifestation of A. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of A. The abscesses ranged from 0. Prior to inoculation, sminis 20 rams were clinically healthy, without palpable lesions in the testes or epididymides.
After centrifugation of cultured A. However, the measurements of the left tail of the epididymides i. The rams were divided into two groups of 10 animals each and the experiments were carried out in Belo Horizonte, Brazil It was confirmed that A. Changes frequently observed include abscess in the left epididymis tail, which was the site of inoculation Figures 5 a and 5 band an increase in size of inguinal and iliac lymph nodes Table 2.
Although both agents are found in the preputial flora of healthy young sheep [ 5 ], A. The Scientific World Journal. Related Posts.
Moustacas,1 Teane M. Silva,1 Luciana F. Santos,1 and Tatiane A. Powell Received26 Aug Published27 Jan Abstract Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. This study aimed to investigate clinical and pathological changes associated with experimental infections with A.
Faushura The values of each point represent the average and standard error. Rabbit antiserum Two New Zealand rabbits weighing 2 kg each were intramuscularly inoculated with A. These results suggest that the kDa protein could be specific to A. The authors declare that there is no conflict of interests regarding the publication of this paper. It is nonmotile and non-sporulated. Urine collection was performed by blocking the breath for 30 seconds.
Abstract Background Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections. Results The multiplex assay was capable of detecting B. The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B.